Intermolecular Specificity of Pancreatic Lipase and the Structural Analysis of Milk Triglycerides

Abstract

Pancreatic lipase released butyrate and palmitate in equimolar quantities from glyceryl l-pal-mitate 2,3-dibutyrate (PBB), an absence of intramolecular specificity. However, when glyceryl l-myristate 2,3-dioleate (MOO) was mixed with PBB, the latter was hydrolyzed more rapidly, indicating inter-molecular specificity. Primary position specificity was maintained during 5-min. digestions when glyceryl 2-butyrate 1,3-dipalmitate was the substrate. Data from the pancreatic lipase digestions of milk fat are presented. Pancreatic lipase digests some classes of milk triglycerides more rapidly than others.