PRODUCTION OF TETANUS TOXIN ON PEPTONE-FREE MEDIA 1

Abstract

Tetanus toxin was produced on a medium free from peptone consisting essentially of a complete acid hydrolysate of casein, a number of accessory growth factors and inorganic salts. An accurately controlled and low conc. of Fe is essential for optimum yields of toxin. The titers obtained are of the same order of magnitude as those produced by the same strain of Clostridium tetani on peptone-infusion media.