RHEUMATOID FACTORS IN PSORIATIC SCALE, SERUM AND CIRCULATING IMMUNE COMPLEXES DETECTED BY AN ISOTYPE‐SPECIFIC ELISA
- 1 November 1987
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Series C: Immunology
- Vol. 95C (1-6), 161-166
- https://doi.org/10.1111/j.1699-0463.1987.tb00025.x
Abstract
An isotype-specific ELISA for detection of rheumatoid factors (RFs) is described. The assay utilizes rabbit anti-albumin IgG bound to albumin as solid phase reactant (test wells). RF-activity was expressed as the difference in reaction between test wells and wells containing F(ab'')2-fragments of the rabbit IgG bound to albumin. The activity correlated well with the conventional Waaler agglutination test on sera that showed a positive Waaler titre, but several sera that were negative by the Waaler test were positive for RF of IgG isotype by the ELISA-test. The method was applied on specimens from psoriatic patients, and RFs of IgG and IgA isotype were demonstrated in serum immune complexes from all the psoriatic patients examined. IgG- and IgA-RF was also demonstrated in extracts from psoriatic scale, and RFs in scale were demonstrated independently of free rheumatoid factor activity in serum.Keywords
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