Procedure to Facilitate Chromosome Counts in Difficult Plant Material
- 1 January 1954
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 29 (5), 261-264
- https://doi.org/10.3109/10520295409115480
Abstract
Numerous mitotic plates of contracted and well-spread chromosomes may be obtained from root tips of plants set on melting ice or snow overnight at room temperature. After 1:3 acetic-alcohol fixation for 0.5 to 3 hours the material is mordanted in a mixture of 7 parts of alcohol plus 21/2 parts of 3% ferric ammonium sulfate for 3 hours to overnight. This solution may be used as storage fluid for flower buds. Deep chromosome coloration without precipitates is secured by staining in a few drops of aceto-carmine for 10–15 minutes after which the tissues are softened by heating in aceto-carmine diluted with 3 parts of 45% acetic acid.Keywords
This publication has 7 references indexed in Scilit:
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