Volume of Rabbit and Rat Renal Cortical Slices Permeable to P-Aminohippurate (PAH), Diodrast and Urokon
- 31 January 1957
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Legacy Content
- Vol. 188 (2), 360-366
- https://doi.org/10.1152/ajplegacy.1957.188.2.360
Abstract
A method is presented which allows one to measure the permeable volume of the renal cortex to substances which are transported or actively accumulated by the renal cortex. This permeable volume in rat and rabbit renal cortical slices has been measured using PAH, Diodrast and Urokon as the transported substances. It is less than the volume of tissue water. Therefore, a volume of the renal cortex must be impermeable to these substances. It is postulated that all or part of the impermeable volume is made up of the tubular cells. The permeable volumes are 0.474 cc/gm tissue for PAH, 0.542 cc/gm tissue for Diodrast, and 0.447 cc/gm tissue for Urokon, as compared to 0.771 cc/gm for the tissue water. The active accumulation of these substances by rabbit renal cortical slices after 90– 130 minutes is 10.10 µm/gm for PAH, after 90 minutes is 1.90 µm/gm for Diodrast, and after 60–150 minutes is 3.20–3.53 µm/gm for Urokon.Keywords
This publication has 8 references indexed in Scilit:
- Effect of Renal Ischemia on Renal Tubular Function in the Rat as Measured by PAH Uptake in VitroAmerican Journal of Physiology-Legacy Content, 1955
- Active Cellular Transport of Urea by Frog Renal TubulesAmerican Journal of Physiology-Legacy Content, 1954
- Iodine Concentration in Rabbit Kidneys After Diodrast InjectionAmerican Journal of Physiology-Legacy Content, 1953
- Historadiographic Demonstration of Diodrast in the Rabbit KidneyAmerican Journal of Physiology-Legacy Content, 1953
- Apparent Volumes of Distribution of p-Aminohippurate and Creatinine in the DogAmerican Journal of Physiology-Legacy Content, 1951
- RENAL TUBULAR TRANSPORT: ACCUMULATION OF P-AMINOHIPPURATE BY RABBIT KIDNEY SLICESAmerican Journal of Physiology-Legacy Content, 1950
- RENAL TUBULAR TRANSPORT: EFFECT OF 2,4-DINITROPHENOL AND RELATED COMPOUNDS ON PHENOL RED TRANSPORT IN THE ISOLATED TUBULES OF THE FLOUNDERAmerican Journal of Physiology-Legacy Content, 1950
- The Volume Distribution and Equilibrium Time of Para-AminohippurateExperimental Biology and Medicine, 1949