Physical Mapping of Temperature-sensitive Mutations of Herpes Simplex Virus Type 2 by Marker Rescue

Abstract

The physical mapping of 6 ts mutations of herpes simplex virus type 2 (HSV-2) is presented. The results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 HSV-1/HSV-2 intertypic recombinants. The order of these mutations on the physical map of HSV-2 is unambiguous and correlates almost exactly with the previously published genetic map of Timbury and Calder. One of the mutants studied (HSV-2 ts 12) apparently has 2 distinct conditionally lethal ts mutations, one in the long and the other in the short region of the HSV genome.