Relationship between serum IgE levels and anaphylactic histamine release from isolated rat mast cells
- 1 December 1979
- journal article
- research article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 107 (4), 313-318
- https://doi.org/10.1111/j.1748-1716.1979.tb06481.x
Abstract
Inbred Hooded Lister rats were immunized with egg albumin with B. pertussis vaccine used as an adjuvant. The serum levels of total IgE and IgE antibody (egg albumin specific) were determined by radioimmunoassay techniques before and after immunization. The basic level of total IgE in serum was 560 ± 110 ng/ml. After immunization a maximal peak at day 11 of 1940 ± 160 ng/ml was registered. Anti‐egg albumin IgE antibody showed a maximum around day 13 of 75 ± 11 units/ml. Pleural mast cells were isolated on Ficoll between day 14 and 20 after immunization. A significant negative correlation between the basic total IgE level and histamine release by antigen (egg albumin) was found and also a significant positive correlation of specific IgE antibody (determined at day 11) and histamine release. The correlation between IgE level and histamine release was slightly improved if instead the ratio of specific IgE antibody over total IgE was used.Keywords
This publication has 21 references indexed in Scilit:
- Effect of sensitization on spontaneous and phosphatidylserine-induced histamine release and on cyclic AMP and GMP levels in isolated rat mast cellsActa Physiologica Scandinavica, 1979
- Analysis of the Serum IgE Levels in Nonimmunized Rats of Various Strains by a RadioimmunoassayScandinavian Journal of Immunology, 1979
- A Radioimmunoassay for Evaluation of the IgE and IgG Antibody Responses in the RatScandinavian Journal of Immunology, 1979
- Evaluation of Basic Serum IgE Levels and the IgE Antibody Response in the Rat by RadioimmunoassaysImmunological Reviews, 1978
- Potentiation of Anaphylactic Histamine Release from Isolated Rat Pleural Mast Cells by Rat Serum PhospholipidsActa Physiologica Scandinavica, 1976
- Half-Lives of Two Types of Rat Homocytotropic Antibodies in Circulation and in the SkinInternational Archives of Allergy and Immunology, 1975
- IMMUNOGLOBULIN E IN BRONCHIAL ASTHMAAllergy, 1974
- Correlation between in vitro Anaphylactic Histamine Release from Tissues and Reagin Titer in RatInternational Archives of Allergy and Immunology, 1974
- A new and simple radioimmunoassay method for the determination of IgEImmunochemistry, 1972
- Degranulation and Histamine Release, Two Consecutive Steps in the Response of Rat Mast Cells to Compound 48/80Acta Physiologica Scandinavica, 1967