Comparative sensitivities of tocopherol levels of platelets, red blood cells, and plasma for estimating vitamin E nutritional status in the rat
- 1 October 1981
- journal article
- research article
- Published by Elsevier in The American Journal of Clinical Nutrition
- Vol. 34 (10), 2104-2110
- https://doi.org/10.1093/ajcn/34.10.2104
Abstract
The use of tocopherol levels of either platelets or red blood cells was tested as an index of tissue vitamin E status. Male Wistar rats were fed 0, 5, 10, 20, or 50 ppm of vitamin E (d-α-tocopheryl acetate) ad libitum for 10 wk. Over the dosage range from 0 to 20 ppm, responses in tocopherol content of most tissues including platelets and red blood cells were linear. Over the whole range from 0 to 50 ppm, responses were curvilinear both directly and logarithmically for all tissues. In a comparison of reproducibility of responses of platelets, plasma and red blood cells, the variation of α-tocopherol content within groups fed the same diet was consistently lowest for platelets. Sensitivity (linear slope/SD) for reflecting vitamin E intake was higher for platelets than for either red blood cells or plasma. In vitro spontaneous hemolysis of red blood cells was less than 10% with 10 ppm of vitamin E, greater than 85% with 0 ppm, and was variable (2 to 78%) with 5 ppm. Lipid levels in plasma increased significantly as vitamin E was increased from 5 to 50 ppm of the diets. Over this range, levels of cholesterol and phospholipid increased 20% and levels of triglyceride increased almost 200%.Keywords
This publication has 18 references indexed in Scilit:
- Effect of different levels of dietary .ALPHA.-tocopherol and linoleate on plasma and liver lipids in rats.Journal of Nutritional Science and Vitaminology, 1978
- Effect of High Levels of Dietary Vitamin E on Liver and Plasma Lipids and Fat Soluble Vitamins in RatsJournal of Nutrition, 1977
- Factors affecting the exchange of tocopherol between red blood cells and plasmaThe American Journal of Clinical Nutrition, 1977
- RELATIONSHIP BETWEEN TOCOPHEROL AND SERUM LIPID LEVELS FOR DETERMINATION OF NUTRITIONAL ADEQUACY*Annals of the New York Academy of Sciences, 1972
- INTAKE AND ABSORPTION OF TOCOPHEROL*Annals of the New York Academy of Sciences, 1972
- Interaction of Dietary Vitamin E and Protein Level or Lipid Source with Serum Cholesterol Level in RatsJournal of Nutrition, 1972
- Blood α‐tocopherol: Erythrocyte and plasma relationships in vitro and in vivoLipids, 1970
- Tocopherol transport in the rat erythrocyteJCI Insight, 1969
- Effect of Antioxidants on Muscle and Plasma Lipids of Vit. E-deficient Guinea Pigs.Experimental Biology and Medicine, 1958
- Sensitivity--a criterion for the comparison of methods of testJournal of Research of the National Bureau of Standards, 1954