NUTRITIONAL REQUIREMENTS AND FACTORS AFFECTING THE PRODUCTION OF TOXIN OF CLOSTRIDIUM SEPTICUM

Abstract

A medium consisting of a complete acid hydrolysate of casein supplemented with cystine, tryptophan, growth accessory factors, glucose, and inorganic salts, has been developed for the cultivation of Clostridium septicum. Toxin equivalent to 400 to 700 L.D₅₀ per ml. has been obtained regularly in this medium. The principal factors found to affect the yield of toxin are:—