IMMUNOLOGICAL STUDIES WITH A COMMERCIAL PREPARATION OF HUMAN CHORIONIC GONADOTROPIN*
Open Access
- 1 April 1963
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 42 (4), 546-555
- https://doi.org/10.1172/jci104743
Abstract
With partially purified, commercial, human chorionic gonadotropin (HCG) as an antigen, antiHCG antibodies have been consistently produced in rabbits and guinea pigs. The antibody has been demonstrated immunologically by hemagglutination and by neutralization of the biological activity of HCG in both the frog and rat. An immunological method for the detection of human chorionic gonadotropin by hemagglutination inhibition has been described. Electrophoretic and immunoelectrophoretic characterization of HCG preparations have been performed and the presence of contaminants has been demonstrated. The effect of adsorbing rabbit HCG antiserum with urine extract indicates that it is not possible to make such antisera specific for HCG.Keywords
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