Defective mitosis due to a mutation in the gene for a fission yeast 26S protease subunit
- 1 December 1993
- journal article
- Published by Springer Nature in Nature
- Vol. 366 (6453), 355-357
- https://doi.org/10.1038/366355a0
Abstract
We have isolated a mutant, mts2, in the fission yeast Schizosaccharomyces pombe which is defective in chromosome segregation. The predicted amino-acid sequence of the cloned mts2+ gene product is 75% identical to the S4 subunit of the human 26S ATP/ubiquitin-dependent protease. The human S4 subunit complementary DNA expressed from an S. pombe expression plasmid can rescue an S. pombe mts2 gene disruption. Both observations demonstrate that the mts2+ gene is the S. pombe homologue of the human S4 subunit. In addition, we provide genetic evidence for a physical interaction between the S4 and the related S7 subunit in the 26S multiprotein protease. We show that polyubiquitin-conjugated proteins accumulate in the mts2 mutant at the restrictive temperature, demonstrating that the mutant has an in vivo defect in the ubiquitin-dependent proteolysis pathway. Finally, the phenotype for the mts2 mutant indicates that protein degradation by the 26S protease is essential not for entry into but for the completion of mitosis.Keywords
This publication has 18 references indexed in Scilit:
- S. cerevisiae 26S protease mutants arrest cell division in G2/metaphaseNature, 1993
- Two fission yeast B-type cyclins, cig2 and Cdc13, have different functions in mitosis.Molecular and Cellular Biology, 1993
- New human gene encoding a positive modulator of HIV Tat-mediated transactivationNature, 1992
- THE UBIQUITIN SYSTEM FOR PROTEIN DEGRADATIONAnnual Review of Biochemistry, 1992
- A fission yeast B-type cyclin functioning early in the cell cycleCell, 1991
- A cyclin B homolog in S. cerevisiae: Chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosisCell, 1991
- The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiaeCell, 1991
- Cyclin is degraded by the ubiquitin pathwayNature, 1991
- The role of cyclin synthesis and degradation in the control of maturation promoting factor activityNature, 1989
- Gene products required for chromosome separationJournal of Cell Science, 1989