STUDIES OF 17-HYDROXYCORTICOSTEROIDS: ADRENALECTOMY AND HYPOPHYSECTOMY IN (UJINEA PK1S AND THEIR EFFECTS ON PLASMA 17-OHCS CONCENTRATIONS1

Abstract

A detailed technique for adrenalectomy and hypophysectomy in guinea pigs is described and the factors which cause operative failure are discussed. Influence of pituitary or adrenal extirpation on circulating plasma 17-OHCS in small and large guinea pigs is presented. Plasma 17-OHCS concentrations fall to zero values within 24 hours following adrenalectomy. After hypophysectomy a rapid decrease in the plasma 17-OHCS concentrations results during the first few hours post-operatively. However, these concentrations do not decrease to zero, but "plateau" at a low level. Prolonged ether anesthesia for the hypophysectomy procedure results in elevated 17-OHCS concentrations 24 hours after operation. Brief ether anesthesia or anesthesia with pentobarbital does not cause such elevations.