Serial Determinations of Serum Glycoproteins Following Antigen Injection into Previously Immunized Animals

Abstract

The effects of antigen administration on the serum concentrations of total glycoprotein, seromucoid, γ-globulin polysaccharide, total protein, and γ-globulin protein have been determined in guinea pigs previously immunized with heatkilled Brucella suis cells. To evaluate the effects of the repeated bleedings normal animals were bled at weekly intervals. Statistically significant increases occurred in all the serum components following antigen administration and remained elevated through the maximal antibody response. Repeated bleedings were found not to be a factor. The results are discussed with reference to possible causes of the increased levels of serum glycoproteins.