Inhibition by fluoride of glucose breakdown in tumour tissue and retina extracts

Abstract

Expts. with tissue from the Crocker tumour and mouse tumour 113 showed that no disappearance of inorganic phosphate occurred in the presence of glucose and NaF. No evidence could be obtained of phosphate ester formation from glucose. The disappearance of glucose in these preps, was inhibited by the addition of NaF. The presence of NaF then, inhibited glucose breakdown at the 1st stage and prevented the formation of any intermediate compounds. Expts. with extracts of pigs'' retinae gave similar results. The disappearance of inorganic phosphate which occurred during glucose glycolysis was inhibited by the presence of NaF, as was also the disappearance of the glucose itself. Dilute NaF (N/1000) inhibited the formation of lactic acid from hexose-diphosphate by tumour tissue and caused the accumulation of some acid other than lactic acid. This did not occur when glucose was used instead of hexose-diphosphate.